Yak lactate dehydgogenase A4: purification, properties, and cDNA cloning.
نویسندگان
چکیده
Lactate dehydrogenase A4 (LDH-A4) was purified for yak skeletal muscle. Michaelis constant (Km) analysis showed that yak LDH-A4 for pyruvate was significantly higher than that of cattle. cDNA cloning of LDH-A revealed two amino acid substitutions between yak and cattle. We suggest that the higher Km of yak LDH-A4 might be a result of molecular adaptation to a hypoxic environment.
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ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 72 9 شماره
صفحات -
تاریخ انتشار 2008